polyclonal goat antibody against transferrin  (Bethyl)

Journal: Lung

Article Title: Soluble Transferrin Receptor-1 in Pulmonary Hypertension Associated with COPD

doi: 10.1007/s00408-025-00833-3

Figure Lengend Snippet: Tissue transferrin receptor-1 in LTX cohort. ( A ) Representative image of IHC staining for CD71 in the lung tissue of healthy donor. Scale: 50 µm. ( B ) Representative image of IHC staining for CD71 in the lung tissue of end-stage COPD patient with no/mild PH. Scale: 50 µm. ( C ) Representative image of IHC staining for CD71 in the lung tissue of end-stage COPD patient with moderate PH. Scale: 50 µm. ( D ) Representative image of IHC staining for CD71 in the lung tissue of end-stage COPD patient with severe PH. Scale: 50 µm. ( E ) Quantification of CD71-positive cells representing transferrin receptor-1 in lung tissues. ( F ) Correlation between the density of CD71-positive cells in the lung and mPAP in COPD. mPAP mean pulmonary artery pressure, V vessel

Article Snippet: Immunohistochemical (IHC) staining for transferrin receptor-1 (CD71) was conducted on formalin-fixed paraffin-embedded tissue sections using a polyclonal goat antibody against CD71 at 1:200 dilution (cat. #AF2474, RRID: AB_416601, R&D Systems, USA), followed by a biotinylated donkey anti-goat secondary IgG antibody (cat. #BA–9500–1.5, Vector Laboratories, USA) and an avidin–biotin complex HRP detection system from the ImmPACT NovaRED® Substrate Kit (cat. #SK-4805, Vector Laboratories, USA), as previously described [ ].

Techniques: Immunohistochemistry